Changes in MicroRNAs Expression and Clinical Associations in Patients with Neuroendocrine Pancreatic Tumors
Context In a previously study, we analyzed associations between altered expression of 13 microRNAs (miR-145, miR-23a, miR-455-3p, miR-708, miR-151-5p, miR-30c, miR-let-7i, miR-199a-5p, miR-30a, miR-143, miR-21, miR-155*, and miR-31) and clinical variables in patients with pancreatic adenocarcinoma. Objective Here, expression levels of these miRNAs were analyzed to evaluate their possible associations with clinical-pathological features in patients underwent surgical resection for pancreatic neuroendocrine tumors (PETs). Methods miRNAs expression levels in tumors compared to matched-pairs non-cancerous tissue samples, were analyzed by using TaqMan MicroRNA Assay. Association analysis was performed by means of Spearman correlation coefficient and Mann-Whitney U-test. Results No significant association was found between miR-145, miR-23a, miR-455-3p, and miR-708 levels and patients sub-phenotypes. Conversely, miR-151-5p expression was inversely correlated with age at diagnosis (rs=-0.77, P=0.009), both miR-30a and miR-let-7i levels were positively associated with tumor size (rs=0.69, P=0.027 and rs=0.67, P=0.034), while over-expression of either miR-199a-5p and miR-30c was associate with male gender (P=0.046 and P=0.003). Further original finding was the association between miR-143, miR-21 and miR-155* levels and tumor mitotic index (P=0.04, P=0.0002, and P<0.0001, respectively), while expression of both miR-31 and miR-30c was associated with tumor proliferation index (P=0.003 and P=0.01, respectively). In addition, miRNAs expression levels were also found to be correlated with each others in PETs tissue samples. Conclusions These data suggest a prognostic significance for specific miRNAs in PETs. Understanding the associations between miRNAs expression levels could help identify novel regulatory network involved biology of PETs.
Copyright (c) 2014 Francesca Tavano, Fabio Francesco di Mola, Francesca Paola Burbaci, Lucia Lombardi, Massimiliano Copetti, Ada Piepoli, Angelo Andriulli, Evaristo Maiello, Pierluigi di Sebastiano
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