Study of Lymphocyte Subpopulations and Cytotoxic Activity in the Blood of Patients Undergoing Surgery for Pancreatic Cancer

  • Francesco Iannone N Unit of General Surgery, “F. Durante” Section of Interdisciplinary Surgery, Department of Surgical Sciences, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Alessandra Porzia Department of Molecular Medicine, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Patrizia Birarelli Department of Experimental Medicine, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Bernardina Milana Department of Experimental Medicine, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Mario Santilli N Unit of General Surgery, “F. Durante” Section of Interdisciplinary Surgery, Department of Surgical Sciences, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Luca Sacco N Unit of General Surgery, “F. Durante” Section of Interdisciplinary Surgery, Department of Surgical Sciences, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Arianna Ripanti N Unit of General Surgery, “F. Durante” Section of Interdisciplinary Surgery, Department of Surgical Sciences, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Giuseppe Dinatale N Unit of General Surgery, “F. Durante” Section of Interdisciplinary Surgery, Department of Surgical Sciences, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Giampaolo Prezioso N Unit of General Surgery, “F. Durante” Section of Interdisciplinary Surgery, Department of Surgical Sciences, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Roberto Caronna N Unit of General Surgery, “F. Durante” Section of Interdisciplinary Surgery, Department of Surgical Sciences, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Stefania Morrone Department of Experimental Medicine, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Fabrizio Mainiero Department of Experimental Medicine, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
  • Piero Chirletti N Unit of General Surgery, “F. Durante” Section of Interdisciplinary Surgery, Department of Surgical Sciences, “Umberto I” Polyclinic, “Sapienza” University of Rome. Rome, Italy
DOI: https://doi.org/10.6092/1590-8577/1750
Keywords: Meeting Abstracts, Pancreas

Abstract

Context The study of the immune responses to pancreatic cancer is an emerging field of clinical and laboratory research since accumulating evidence indicate that the lymphocyte count, the Th2 polarization, the profile of cytokines and chemokines produced by cancer and immune cells and the presence of CD8+ tumor-infiltrating lymphocytes (TILs) correlate with the tumor prognosis. Objective The purpose of our study is to analyze the lymphocyte population distribution and natural killer (NK) cell cytotoxic activity before and after curative pancreatic resection (radical exeresis of mesopancreas and extended lymphadenectomy). Methods We collected blood samples from 17 pancreatic cancer patients at three different time points, before surgery and 7 and 30 days after surgery, and we evaluated the peripheral blood lymphocyte profile by cytofluorimetric analysis and the NK cytotoxic activity by a 51chromium release assay. Seventeen blood samples from healthy subjects were used as control. Results The analysis of five lymphocyte subpopulations (T CD3+, T CD4+, T CD8+, NK CD56+, and B CD19+ cells) reveals a statistically significant increase (P=0.04) of NK CD56+ cells between the blood samples of patients, but not of controls, collected before and 30 days after surgery. Moreover, we observed a correlated modulation of NK cytotoxic activity. A more detailed classification of cancer pancreatic patients based on TNM and histological type revealed a further statistically significant modulation of T CD3+, T CD4+ and B CD19+ lymphocyte subpopulations and of NK cytotoxic activity. Conclusions Data show the importance of a radical pancreatic resection with complete mesopancreas removal and extended lymphadenectomy in the resumption of the NK cells and in the modulation of other lymphocyte subpopulations which may affect pancreatic cancer prognosis.

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AISP 37th National Congress
Published
2013-09-15
How to Cite
IannoneF., PorziaA., BirarelliP., MilanaB., SantilliM., SaccoL., RipantiA., DinataleG., PreziosoG., CaronnaR., MorroneS., MainieroF., & ChirlettiP. (2013). Study of Lymphocyte Subpopulations and Cytotoxic Activity in the Blood of Patients Undergoing Surgery for Pancreatic Cancer. JOP. Journal of the Pancreas, 14(5S), 576. https://doi.org/10.6092/1590-8577/1750
Issue
Vol 14 No 5S (2013): September (Suppl.) - p. 528-602
Section
Poster Session

Copyright (c) 2014 Francesco Iannone, Alessandra Porzia, Patrizia Birarelli, Bernardina Milana, Mario Santilli, Luca Sacco, Arianna Ripanti, Giuseppe Dinatale, Giampaolo Prezioso, Roberto Caronna, Stefania Morrone, Fabrizio Mainiero, Piero Chirletti

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