[6]-Shogaol Induces Ca2+ Signals by Activating the TRPV1 Channels in the Rat Insulinoma INS-1E Cells

Paola Rebellato; Md. Shahidul Islam

doi:10.6092/1590-8577/1979

Paola Rebellato Department of Clinical Science and Education, Södersjukhuset, Research Centre and Department of Medical Biochemistry and Biophysics; Karolinska Institutet. Stockholm, Sweden
Md. Shahidul Islam Department of Clinical Science and Education, Södersjukhuset, Research Centre, Karolinska Institutet. Stockholm, Sweden. Department of Internal Medicine, Uppsala University Hospital. Uppsala, Sweden

DOI: https://doi.org/10.6092/1590-8577/1979

Keywords: Calcium Signaling, Insulin-Secreting Cells, Islets of Langerhans, shogaol, TRPV1 receptor

Abstract

Context [6]-shogaol is a vanilloid compound present in steamed ginger (Zingiber officinale), a commonly used spice. Pancreatic beta-cells respond to nutrients like glucose, amino acids and fatty acids, by an increase in the cytoplasmic free Ca²⁺ concentration ([Ca²⁺]_i), which mediates diverse cellular processes in these cells. Some vanilloid compounds activate the transient receptor potential vanilloid receptor type 1 (TRPV1) channel. Objective We investigated whether [6]-shogaol could trigger Ca²⁺ signals in the beta-cell. Methods [Ca²⁺]_i was measured from single INS-1E cells by microscope-based fluorometry using fura-2 as the Ca²⁺ indicator. Results In fura-2 loaded single rat insulinoma INS-1E cells, a widely used model of beta-cell, [6]-shogaol increased [Ca²⁺]_i in a concentration-dependent manner. [Ca²⁺]_i increase by [6]-shogaol was completely blocked when Ca²⁺ was omitted from the extracellular medium. Capsazepine, an inhibitor of the TRPV1 ion channel completely inhibited the [6]-shogaol-induced [Ca²⁺]_i increase. [Ca²⁺]_i increase obtained by 1 µM [6]-shogaol was greater than that obtained by 10 mM glucose. Moreover, a sub-stimulatory concentration of [6]-shogaol (300 nM), significantly enhanced the glucose-induced [Ca²⁺]_i increase in these cells. Conclusion We conclude that [6]-shogaol induces Ca²⁺ signals in the beta-cell by activating the TRPV1 channels, and it sensitizes the beta-cells to stimulation by glucose.

Image: [6]-shogaol-induced [Ca2+]i increase.

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Author Biography

Md. Shahidul Islam, Department of Clinical Science and Education, Södersjukhuset, Research Centre, Karolinska Institutet. Stockholm, Sweden. Department of Internal Medicine, Uppsala University Hospital. Uppsala, Sweden

Senior Consultant Physician, Department of Internal medicine, Uppsala University Hospital.

Associate Professor, Karolinska Institutet, Stockholm, Sweden

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