Effect of Livingstone Potato (Plectranthus esculentus N.E.Br) on Hepatic Glucose-6-Phosphophate Dehydrogenase Activity of Streptozotocin Induced Diabetic Rats

  • Eleazu Chinedum Ogbonnaya National Root Crops Research Institute. Umudike, Nigeria
Keywords: Antioxidants, Glucosephosphate Dehydrogenase, Polyphenols


Context To determine the effect of Livingstone potato (Plectranthus esculentus N.E.Br) on hepatic G6PD in streptozotocin induced diabetic rats. Methods The G6PD activities in the liver homogenates of the rats and chemical analysis of the test feeds were determined using standard techniques. Results The diabetic control rats had significant elevation (P < 0.05) of their hepatic G6PD activities compared with the non-diabetic rats. Intake of the test feed by the diabetic rats of group 3, resulted in significant (P<0.05) amelioration of their hepatic G6PD activities in comparison with the diabetic control rats. Chemical analysis of the test feed revealed that it contained considerable amounts of tannins, saponins, alkaloids, antioxidants and their cyanide contents were below the toxic level for humans. Conclusion The study shows ameliorative potentials of Livingstone potato on the hepatic G6PD activity of diabetic rats which is attributed to its antioxidant/polyphenolic constituents.

Image: Reducing power of test feed and standard quercetin.


Download data is not yet available.


McAnuff MA, Omoruyi FO, Morrison EY, Asemota HN. Changes in Some Liver Enzymes in Streptozotocin-induced Diabetic Rats fed Sapogenin Extract from Bitter Yam (Dioscorea polygonoides) or Commercial Diosgenin. West Indian Med J. 2005; 54: 97. [PMID: 15999877]

Robert KM, Darry KG, Peter AM. Harper’s Biochemistry, 23rd Edition. Appleton and Lange, USA, 1993; 150-151. [PMID:6039601]

Jablonska-Skwiecinska E, Lewandowska I, Plochocka D, Topczewski J, Zimowski JG, Klopocka J, Burzynska B. Several mutations including two novel mutations of the glucose-6-phosphate deficient subjects with chronic nonspherocytic hemolytic anemia and favism. Hum Mutat. 1999; 14: 477-484. [PMID:10571945]

Yadav UC, Moorthy K, Baquer NZ. Effects of Sodium-Orthovanadate and Trigonella Foenum-Graecum Seeds On Hepatic And renal lipogenic enzymes and lipid profile during alloxan diabetes. J Biosci. 2004; 29: 12. [PMID:15286407]

Debidas G,Tushar KK, Chatterjee K, Debasis D. Anti-diabetic and anti-oxidative effects of aqueous extract of seed of psoralea Corylifolia (somraji) and seed of trigonella foenum-graecum in separate and composite manner in streptozotocin-induced diabetic male albino rat. Int J Pharm Res Develop. 2009; 7: 976-984.

Eleazu, C.O., Eleazu, K.C., Chukwuma, S.C., Ironkwe, C., Iroaganachi, M.A. and Emelike, C.U. Effect of Livingstone potato (Plectranthus esculentus N.E.Br) on diabetes and its complications in Streptozotocin induced diabetes in rats. DMJ Diab Metabol J. 2014.

Gomathi D, Ravikumar G, Kalaiselvi M, Devaki K, Uma C. Efficacy of Evolvulus alsinoides (L.) L. on insulin and antioxidants activity in pancreas of streptozotocin induced diabetic rats. J Diabetes Metab Disord. 2013; 12:39. [PMID:23834750]

National Research Council (NRC). Guide for the care and use of laboratory animals. Publication 8523, National Institute of Health, Bethesda, Md, USA, 1985.

Singh SN, Vats P, Suri S, Shyam R, Kumria MM, Ranganathan S, Sridharan K. Effect of an antidiabetic extract of Catharanthus roseus on enzymic activities in streptozotocin induced diabetic rats. J Ethnopharmacol. 2001; 76: 269-277. [PMID:11448549]

Noltmann EA, Gubler CJ, Kuby SA. Glucose 6-phosphate dehydrogenase (Zwischenferment). I. Isolation of the crystalline enzyme from yeast. J Biol Chem 1961; 236:1225-1230. [PMID:13729473]

Blois MS. Antioxidant determination by use of stable free radicals. Nature. 1985; 29:1199-1200.

Hsu CL, Chen W, Weng YM, Tseng CY. Chemical composition, physical properties, and antioxidant activities of yam flours as affected by different drying methods. Food Chem. 2003; 83: 85-92.

Harbone JB. Comparative Biochemistry of the Flavonoids. New York Academic Press 1973; 221-222.

Boham AB, Kocipai AC. Flavonoid and condensed tannins from leaves of Hawaiian vaccininum vaticulum and vicalycinium. Pacific Sci. 1994; 48: 458-463.

Onwuka GI. Food Analysis and Instrumentation. Theory and Practice. Napthali Prints 2005; 140-146.

AOAC. Official Methods of Analysis, 15th edn. Association of Official Analytical Chemists, Arlington, VA. 1990.

Gupta D, Raju J, Prakash J, Baquer NZ. Change in the lipid profile, lipogenic and related enzymes in the livers of experimental diabetic rats: effect of insulin and vanadate. Diabetes Res Clin Pract. 1999; 46: 1-7. [PMID:10580609]

Raju J, Gupta D, Rao AR, Yadava PK, Baquer NZ. Trigonella foenum-graecum (fenugreek) seed powder improves glucose homeostasis in alloxan diabetic rat tissues by reversing the altered glycolytic, gluconeogenic and lipogenic enzymes. Mol Cell Biochem. 2001; 229: 22445-22451. [PMID:11693199]

Bahadoran Z, Mirmiran P, Azizi F. Dietary polyphenols as potential nutraceuticals in management of diabetes: a review. J Diabetes Metab Disord. 2013; 12: 43. [PMID:23938049]

Shin SH. Oxidative stress and diabetic vascular complications. Recent Advances In Pathogenesis and Management of Diabetes Mellitus. 1st edn. Elsvier Science Company, Singapore. 1998; 633: 3-8.

Scalbert A, Johnson IT, Saltmarsh M. Polyphenols: antioxidants and beyond. Am J Clin Nutr 2005; 81: 215S-217S. [PMID:15640483]

Price KR, Johnson IT, Fenwic GR. The chemical and biological significance of saponins in food and feeding stuff. Crit Rev Food Sci Nutr. 1987; 26: 27-135. [PMID:3308321]

FAO/IFAD. The World Cassava Economy: Facts, Trends and Outlooks. Food and Agriculture Organization of the United Nations and International Fund for Agricultural Development, Rome. 2000.

Bolhius GG. The toxicity of cassava roots. Neth J Agric. 1954; 2: 176-185.

Akiyama H, Toida T, et al . Determination of cyanide and thiocyanate in Sugihirata Ke Mushroom using HPLC method with fluorimetric detection. J Health Sci. 2006; 52: 73-77.

Choong CT, Van-Den T, Roger FM, Roger LT, Kenneth VP, Craig Y. Antioxidant activities, phenolic and β-carotene contents of sweet potato genotypes with varying flesh colours. Food Chem. 2007; 103: 829-838.

Parameswari G, Suriyavathana M. Evaluation of total phenolic content and free radical scavenging activity of Boerhavia erecta. J Acute Med 2013; 2: 1-7.

Reducing power of test feed and standard quercetin.
How to Cite
OgbonnayaE. (2014). Effect of Livingstone Potato (Plectranthus esculentus N.E.Br) on Hepatic Glucose-6-Phosphophate Dehydrogenase Activity of Streptozotocin Induced Diabetic Rats. JOP. Journal of the Pancreas, 15(4), 360-364. https://doi.org/10.6092/1590-8577/2573