Isolated Chick B islets

Response of Chick B Islets to Insulin Secretagogues is Comparable to those of Human Islet Equivalents

Bhawna Chandravanshi, Savita Datar, Ramesh Bhonde

Abstract


Context The B islets isolated from 3-5 day old chick respond well to glucose challenge in a similar fashion to those isolated from mouse pancreas. Objective To compare insulin secretory response of chick B islets with that of human islet equivalents generated from stem cells. Methods Human umbilical cord mesenchymal stem cells were differentiated into hIEqs employing three step sequential serum free protocols. Results Immunofluorescence staining demonstrated insulin, C peptide and Glut 2 positivity of both these islets. Static insulin stimulation of these islets in response to glucose, metformin and gamma amino butyric acid resulted in increased insulin secretion as compared to basal glucose stimulation. Our results demonstrate that insulin secretory response of Chick B islets to metformin and gama amino butyric acid is comparable to those of hIEqs. Moreover, both chick and hIEqs could be successfully cryopreserved and revived in a commercially available cryomix (Cryostore 5®), indicating resemblance in their behavior at sub-zero temperatures. Conclusion Present study advocates Chick islets as an alternative source for diabetes research and islet banking.

Image: Isolated Chick B islets.


Keywords


Cryopreservation; Mesenchymal Stromal Cells; Umbilical Cord

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References


Defronzo RA, Ferrannini E, Simonson DC. Fasting hyperglycemia in non-insulin dependent diabetes mellitus: contributions of excessive hepatic glucose production and impaired glucose uptake. Metabolism 1989; 38:387-395. [PMID: 2657323]

Defronzo RA. Pathogenesis of type 2 (Non-insulin dependent) diabetes mellitus: a balanced overview. Diabetologia 1992; 34:607-610. [PMID: 1516769]

Del prato S, Bonnadonna RC, Bonora E, Gulli G, Solini A, Shank M, Defronzo RA. Characterization of cellular defects of insulin action in type 2 (non-insulin dependent) diabetes mellitus. J Clin Invest 1993; 9:484-494. [PMID: 8432857]

Krinsley JS, Schultz MJ, Spronk PE, Harmsen RE, van Braam Houckgeest F, van der Sluijs JP, et al. Mild hypoglycemia is independently associated with increased mortality in the critically ill. Crit Care 2011; 15:R173. [PMID: 21787410]

Cryer PE, Davis SN, Shamoon H. Hypoglycemia in diabetes. Diabetes Care 2003; 26:1902-12. [PMID: 12766131]

Kuzuya T, Nakagawa S, Satoh J, Kanazawa Y, Iwamoto Y, Kobayashi M, Nanjo K, et al. Report of the Committee on classification and diagnostic criteria of diabetes mellitus. Diabetes Res. Clin Pract 2002; 55:65-85. [PMID: 11755481]

Cryer PE. Mechanisms of Hypoglycemia-Associated Autonomic Failure and Its Component Syndromes in Diabetes. Diabetes 2005; 54:3592-601. [PMID: 16306382]

Constantino MI, Molyneaux L, Limacher-Gisler F, Al-Saeed A, Luo C, et al. Long-term complications and mortality in young-onset diabetes: type 2 diabetes is more hazardous and lethal than type 1 diabetes. Diabetes Care 2013; 36:3863-3869. [PMID: 23846814]

Lloyd A, Sawyer W, Hopkinson P. Impact of long-term complications on quality of life in patients with type 2 diabetes not using insulin. Value Health 2001; 4:392-400. [PMID: 11705130]

Datar SP and Bhonde RR. A simple technique for isolation of functional B- islets from chick pancreas. Journal of Cell and Tissue Research 2006; 6:605-608.

Modak MA, Datar SP , Bhonde RR, Ghaskadbi SS. Differential susceptibility of chick and mouse islets to streptozotocin and its co-relation with islet antioxidant status. J Comp Physiol B 2007; 177:247-57. [PMID: 17205303]

Flotzer C, Haffen K, Kedinger M, Mialhe P. Simulation of insulin and glucagons secretion in organ culture of chick endocrine pancreas during embryonic life and after hatching. Gen Comp Endocrinol 1982; 47:213-220. [PMID: 6124484]

Simon J, Dubois MP. Failure of a sublethal streptozotocin dose to induce diabetes in the chicken. Horm Metab Res 1980; 12:631-632. [PMID: 6450722]

Datar SP, Suryavanshi DS, and Bhonde RR. Chick Pancreatic B Islets as an Alternative in Vitro Model for Screening Insulin Secretagogues. Poultry Science 2006; 85:2260-2264. [PMID: 17135684]

Kadam SS, Bhonde RR. Islet neogenesis from the constitutively nestin expressing human umbilical cord matrix derived mesenchymal stem cells. Islets 2010; 2:1-9. [PMID: 21099303]

Samual AC, Kermit MB, Sandra SD. Staining and in vitro toxicity of dithiozone with canine porcine and bovine islets. Cell Transplant 1994; 3:299-306. [PMID: 7522865]

Danby R, BluV L, Deheny TP, Gibson WR. Effects of alloxan and streptozotocin at high doses on blood glucose levels, glucose tolerance, and responsiveness to sulphonylureas in chicks. Gen Comp Endocrinol 1982; 47:159-169. [PMID: 6212282]

Stellenwerf WA Jr, Hazelwood RL. Peripheral utilization of a glucose load after alloxan and streptozotocin in the rat and chick: a comparison. Gen Comp Endocrinol 1979; 39:131-140. [PMID: 159201]

Nam KH, Yong W, Harvat T, Adewola A, Wang S, Oberholzer J, EddingtonDT. Size-based separation and collection of mouse pancreatic islets for functional analysis. Biomed Microdevices 2010; 12:865-74. [PMID: 20549367]

Bonner-Weir S, Weir G. The organization of the endocrine pancreas: A hypothetical unifying view of the phylogenetic difference. Gen Comp Endocrinol 1979; 38:28-37. [PMID: 381098]

Weir GC, Goltos PC, Steinberg EP, Patel YC. Concentration of Somatostatin immunore activity in chicken pancreas. Diabetologia 1976; 12:129-132. [PMID: 773728]

Ruffier L, Simon J, and Rideau N. Isolation of functional glucagon islets of Langerhans from the chicken pancreas. Gen Comp Endocrinol 1998; 112:153-162. [PMID: 9784298]

Cabrera O, Berman DM, Kenyon NS, Ricordi C, Berggren PO, Caicedo A. The unique cytoarchitecture of human pancreatic islets has implications for islet cell function. Proc Natl Acad Sci USA 2006; 103:2334-2339. [PMID: 16461897]

Kakimoto T, Kimata H, Iwasaki S, Fukunari A, Utsumi H. Automated recognition and quantification of pancreatic islets in Zucker diabetic fatty rats treated with exendin-4. Journal of Endocrinology 2013; 216: 13-20. [PMID: 23092878]

Lupi R1, Del Guerra S, Fierabracci V, Marselli L, Novelli M, Patanè G, et al. Lipotoxicity in human pancreatic islets and the protective effect of metformin. Diabetes 2002; 1:S134-7. [PMID: 11815472]

Lupi R, Del Guerra S, Tellini C, Giannarelli R, Coppelli A, Lorenzetti M, Carmellini M, et al. The biguanide compound metformin prevents desensitization of human pancreatic islets induced by high glucose. Eur J Pharmacol 1999; 364:205-9. [PMID: 9932725]

Prud'homme GJ, Glinka Y, Hasilo C, Paraskevas S, Li X, Wang Q. GABA protects human islet cells against the deleterious effects of immunosuppressive drugs and exerts immunoinhibitory effects alone. Transplantation 2013; 96:616-23. [PMID: 3851932]




DOI: http://dx.doi.org/10.6092/1590-8577/3006

NBN: http://nbn.depositolegale.it/urn%3Anbn%3Ait%3Aunina-14176

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