Gα15 Potential as a Biomarker for Pancreatic Carcinoma

  • Giulio Innamorati Laboratory of Translational Surgery, LURM, University of Verona. Verona, Italy
  • Maria Teresa Valenti Department of Medicine, University of Verona. Verona, Italy
  • Luca Dalle Carbonare Department of Medicine, University of Verona. Verona, Italy
  • Filippo Perbellini Department of Medicine, University of Verona. Verona, Italy
  • Francesco Giovinazzo Department of Medicine, University of Verona. Verona, Italy
  • Sara Zanini Laboratory of Translational Surgery, LURM, University of Verona. Verona, Italy
  • Giorgio Malpeli Department of Pathology and Diagnostic, University of Verona. Verona, Italy
  • Stefano Barbi Department of Pathology and Diagnostic, University of Verona. Verona, Italy
  • Claudio Bassi Laboratory of Translational Surgery, LURM, University of Verona. Verona, Italy
DOI: https://doi.org/10.6092/1590-8577/966
Keywords: Meeting Abstracts, Pancreas

Abstract

Context Pancreatic carcinoma still lacks specific markers. Earlier diagnosis could benefit many patients anticipating resection or other potential cures that today have palliative effects. In addition, differential diagnosis of suspect lesions obtained by imaging could spare resections of benign tumors. Here, we analyzed Gα15 as a potential diagnostic marker. Gα15 is an intracellular signaling protein that we previously found to be ectopically expressed in the pancreas upon transformation. Objective To establish a correlation between the presence of malignant cells in human pancreas and Gα15 mRNA expression in biopsies and in the blood. Methods Microarray studies available in Oncomine analyzing pancreatic cancer were selected; all data sets including human pancreatic cancer cases paired to adjacent healthy tissue were taken in consideration, for a total of 54 pairs of samples. Furthermore, free RNA was extracted from serum of patients affected by pancreatic cancer. First-strand cDNA was generated retrotranscribing with random hexamers. RT PCR was performed using TaqMan gene-specific primers and probe specific for Gα15 sequence. Results All three microarrays datasets, normalized to compare neoplastic to normal tissues, report Gα15 overexpression in neoplastic tissue with higher Gα15 mRNA levels in 49/54 patients. The presence of circulating Gα15 mRNA was assessed in the serum of 8 adenocarcinoma patients showing a significant increase over samples obtained from 8 healthy donors. Conclusions Gα15 mRNA appearance in neoplastic pancreas or in the blood stream should be explored to assess its potential as an indicator of the presence of malignant cells.

 

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Published
2012-09-20
How to Cite
InnamoratiG., ValentiM. T., Dalle CarbonareL., PerbelliniF., GiovinazzoF., ZaniniS., MalpeliG., BarbiS., & BassiC. (2012). Gα15 Potential as a Biomarker for Pancreatic Carcinoma. JOP. Journal of the Pancreas, 13(5S), 565. https://doi.org/10.6092/1590-8577/966
Issue
Vol 13 No 5S (2012): September (Suppl.) - p. 548-650
Section
Oral Communications

Copyright (c) 2014 Giulio Innamorati, Maria Teresa Valenti, Luca Dalle Carbonare, Filippo Perbellini, Francesco Giovinazzo, Sara Zanini, Giorgio Malpeli, Stefano Barbi, Claudio Bassi

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