Pancreatic Peptides in Young and Elderly Zucker Type 2 Diabetic Fatty Rats
Abstract
Context The global prevalence of diabetes mellitus, and in particular type 2 diabetes mellitus is increasing at an alarming rate. Risk factors for development of diabetes include obesity and advancing age. Objectives The distribution of insulin, glucagon, somatostatin and pancreatic polypeptide in the pancreatic islets has been investigated in young and elderly type 2 Zucker diabetic fatty (ZDF) rats and age-matched Zucker lean (ZL) controls. Methods Experiments were performed in male animals aged either 9-13 weeks or 30-34 weeks. Immunohistochemistry was used to label insulin, glucagon, somatostatin and pancreatic polypeptide in islet cells. Results The percentage of insulin-positive cells was unaltered in young but decreased in elderly ZDF (35.5±2.5%) rats compared to ZL controls (57.9±1.8%). The percentage of glucagon-positive cells was increased in young ZDF (58.7±3.4%) compared to ZL controls (23.4±2.1%). However, in elderly rats the percentage of glucagon-positive cells declined in ZDF rats and was no longer different from ZL controls. The percentage of somatostatin-positive cells was unaltered in young and elderly ZDF rats compared to ZL controls. The percentage of pancreatic polypeptide-positive cells was unaltered in young but increased in elderly ZDF (22.0±2.5%) rats compared to ZL controls (13.8±1.8%). Conclusions The distribution of pancreatic hormones is altered to varying extents in the ZDF rat and during the normal aging process.
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References
Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004; 27:1047-53. [PMID 15111519]
Winer N, Sowers JR. Epidemiology of diabetes. J Clin Pharmacol 2004; 44:397-405. [PMID 15051748]
Guillausseau PJ, Laloi-Michelin M. Pathogenesis of type 2 diabetes mellitus. Rev Med Interne 2003; 24:730-7. [PMID 14604750]
Burcelin R, Knauf C, Cani PD. Pancreatic alpha-cell dysfunction in diabetes. Diabetes Metab 2008; 34(Suppl 2):S49-55. [PMID 18640586]
Adeghate E, Donath T. Morphometric and immuno¬histochemical study on the endocrine cells of pancreatic transplants. Exp Clin Endocrinol 1991; 98:193-9. [PMID 1685711]
Torres TP, Catlin RL, Chan R, Fujimoto Y, Sasaki N, Printz RL, et al. Restoration of hepatic glucokinase expression corrects hepatic glucose flux and normalizes plasma glucose in zucker diabetic fatty rats. Diabetes 2009; 58:78-86. [PMID 18952838]
Soria B, Andreu E, Berna G, Fuentes E, Gil A, Leon-Quinto T, et al. Engineering pancreatic islets. Pflugers Arch 2000; 440:1-18. [PMID 10863992]
Wieczorek G, Pospischil A, Perentes E. A comparative immunohistochemical study of pancreatic islets in laboratory animals (rats, dogs, minipigs, nonhuman primates). Exp Toxicol Pathol 1998; 50:151-72. [PMID 9681646]
Lonovics J, Devitt P, Watson LC, Rayford PL, Thompson JC. Pancreatic polypeptide. A review. Arch Surg 1981; 116:1256-64. [PMID 7025798]
Engelking LR. Physiology of the endocrine pancreas. Semin Vet Med Surg (Small Anim) 1997; 12:224-9. [PMID 10889869]
Hiramatsu S, Inoue K, Sako Y, Umeda F, Nawata H. Secretion of insulin and glucagon by the perfused pancreas of genetically obese (fa/fa) Zucker rats and its alteration with aging. Endocr J 1995; 42:563-7. [PMID 8556065]
Akehi Y, Oketa A, Mitsuyoshi Y, Yoshida R, Ohkubo K, Yamashita T, et al. The effects of age on insulin sensitivity and insulin secretion in Japanese subjects with normal glucose tolerance. Rinsho Byori 2007; 55:901-5. [PMID 18050666]
Fritsche A, Stumvoll M, Haring HU. Insulin secretion and aging. Implications for insulin therapy in the geriatric patient with diabetes mellitus. Med Klin (Munich) 2003; 98:287-91. [PMID 12721675]
Riccillo FL, Bracamonte MI, Console GM, Gomez Dumm CL. Histomorphological and quantitative immunohistochemical changes in the rat pancreas during aging. Biocell 2004; 28:127-34. [PMID 15462563]
Janssen SW, Hermus AR, Lange WP, Knijnenburg Q, van der Laak JA, Sweep CG, et al. Progressive histopathological changes in pancreatic islets of Zucker Diabetic Fatty rats. Exp Clin Endocrinol Diabetes 2001; 109:273-82. [PMID 11507651]
Adeghate E, Parvez SH. Nitric oxide and neuronal and pancreatic beta cell death. Toxicology 2000; 153:143-56. [PMID 11090953]
Ausma J, Cleutjens J, Thone F, Flameng W, Ramaekers F, Borgers M. Chronic hibernating myocardium - interstitial changes. Mol Cel Biochem 1995; 147:35-42. [PMID 7494552]
Ahmed I, Adeghate E, Sharma AK, Pallot DJ, Singh J. Effects of Momordica charantia fruit juice on islet morphology in the pancreas of the streptozotocin-diabetic rat. Diabetes Res Clin Pract 1998; 40:145-51. [PMID 9716917]
Howarth FC, Marzouqi FM, Al Saeedi AM, Hameed RS, Adeghate E. The effect of a heavy exercise program on the distribution of pancreatic hormones in the streptozotocin-induced diabetic rat. JOP. J Pancreas (Online) 2009; 10:485-91. [PMID 19734622]
Ueno N, Asakawa A, Satoh Y, Inui A. Increased circulating cholecystokinin contributes to anorexia and anxiety behavior in mice overexpressing pancreatic polypeptide. Regul Pept 2007; 141:8-11. [PMID 17306894]
Copyright (c) 2014 Frank Christopher Howarth, Mey Khalfan Ahmed Ali Al Kitbi, Rasheed Shaul Hameed, Ernest Adeghate
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